briannalp
briannalp briannalp
  • 04-09-2018
  • Biology
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how do scanning electron microscopes differ from transmission microscopes

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mazingmay mazingmay
  • 04-09-2018
The accelerating voltages are much lower than in TEM because it is no longer necessary to penetrate the specimen; in a SEM they range from 50 to 30,000 volts;

as well as rather than the broad static beam used in TEM, the SEM beam is focused to a fine point and scans line by line over the sample surface in a rectangular raster pattern
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